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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: AliD on July 26, 2016, 04:43:12 AM

Title: Glucose solution and refractometer
Post by: AliD on July 26, 2016, 04:43:12 AM
Hi,

I hope this is the right place to put this.  I made up a 63 % glucose solution by dissolving 630 g (dry weight) dextrose powder in a final volume of 1L.  My supervisor wanted me to confirm the solution strength by measuring it on a glucose refractometer.  When I did so the refractometer gave me a reading of 43 %.  I also checked on a YSI glucose analyser which gave me a reading of 70 %.  Can anyone explain why I'm seeing such differences?
Title: Re: Glucose solution and refractometer
Post by: Mitch on July 26, 2016, 01:35:14 PM
Have you calibrated the instrument against commercial standards?