Chemical Forums
Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: HailoMan on September 26, 2016, 12:49:52 AM
-
Apology in advanced if this is a silly question, but in calibrating 1H NMR spectra, can I use water residue as a reference instead of the internal solvent residue?
I did a 1H NMR measurement of gamma-valerolactone (GVL) in DMSO-d6. The DMSO residue peak (2.50 ppm) happen to overlap with 2 of the proton peaks of GVL, so I can't use the DMSO as an internal standard.
-
Surely this compound should be soluble in chloroform? Then you can use that as the reference.
-
The chemical shift of water depends upon temperature; therefore, one would have to take this into account.
-
Surely this compound should be soluble in chloroform? Then you can use that as the reference.
Unfortunately, the compound (nitrogen-doped carbon nanotubes) is non-dispersible in chloroform or other common NMR solvents.
The chemical shift of water depends upon temperature; therefore, one would have to take this into account.
Thank you for this information. I will look into it.
-
Why not just add an internal reference that does not overlap with anything - e.g. tetramethylsilane at 0 ppm?