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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: mmrn on January 17, 2017, 09:00:57 AM

Title: How to discover lukewarm (LKW) leucine concentration
Post by: mmrn on January 17, 2017, 09:00:57 AM

Hi!!
In the lab I'm working there is a vial containing a lukewarm (LKW) leucine solution that was given from another lab, but I don't have any information about the concentration or anything! I asked around and no one had this written. Is that a way to find out the concentration?  ???
I don't know if it helps but the lab has a scintillation counter. I'll need this to measure bacterial production by the ³H-leucine incorporation into proteins.

Title: Re: How to discover lukewarm (LKW) leucine concentration
Post by: Babcock_Hall on January 17, 2017, 09:44:25 AM

I assume that leucine is the only amino acid present.  If you need the concentration of leucine, then you might be able to obtain it colorimetrically, with a quantitative ninhydrin assay.  The other possibility that comes to mind is to analyze for nitrogen in some way.  However, I don't know how the presence of tritium changes the practicality of either experiment.