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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Jdevault@gpalab.com on October 24, 2017, 03:15:07 PM

Title: Need Beta Glucan Extraction Help
Post by: Jdevault@gpalab.com on October 24, 2017, 03:15:07 PM

I am trying to find a way to extract Beta Glucan from grains such that the viscosity is low enough to allow running on HPLC.  If anyone could point me in the right direction, it would be appreciated!

Title: Re: Need Beta Glucan Extraction Help
Post by: KLITE on December 09, 2017, 01:23:05 PM

Hey

I say use oat bran and do it through enzymatic process. To avoid viscosity perhaps some kind of digestive enzyme? I think alpha amylase will play an important role, maybe lipase to improve solubility? Some form of protease might help?

Good luck man!