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Topic: Standard curve for albumin  (Read 3735 times)

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Offline seanifred

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Standard curve for albumin
« on: October 10, 2007, 11:01:42 PM »
So I  have a question on my biochem lab that shows the me standard curve (spectrophotometry) for albumin at 540 nm.  The actual question is for me to sketch the curve obtained for the same sample that has absorbance measured at 600 nm.  How do these relate (I think it has to do with the molar absorptivity but I don't know!!)

Thank you  :)
Chemistry B.S. UCD 2009
Physical Chemistry PhD UCD in progress

Offline ARGOS++

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Re: Standard curve for albumin
« Reply #1 on: October 11, 2007, 03:37:04 PM »

Dear Seanifred,

For the identical sample in UV/Vis spectroscopy you can use the “Special Beer-Lambert Law” and set for both wavelength:
 
Aλ1 = ελ1 * c * d   AND:   Aλ2 = ελ2 * c * d   
 
As for the identical Sample c and d in both cases are identical you will end in
 
ελ2 = ελ1 * Aλ2 / Aλ1


I hope it may be of help.

Good Luck!
                    ARGOS++

P.S.: I always thought that albumin is derived from “white”, what should introduce, that in the range 500 nm till 700 nm you should NOT find any Absorption!
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