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Author Topic: composition and use of KREBS BUFFER SOLUTION ???  (Read 26643 times)

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najam696

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composition and use of KREBS BUFFER SOLUTION ???
« on: December 04, 2007, 12:16:26 AM »

Can any one explain whats KREBS BUFFER SOLUTION is and what is its composition is ???
thanx in advance .
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AWK

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Re: composition and use of KREBS BUFFER SOLUTION ???
« Reply #1 on: December 04, 2007, 02:16:31 AM »

Different formulation are used depending on pH and osmotic pressure,eg

Krebs-Ringer buffer (115 mM NaCl, 5.9 mM KCl, 1.2 mM MgCl2, 1.2 mM NaH2PO4, 1.2 mM Na2SO4, 2.5 mM CaCl2, 25 mM NaHCO3, 10 mM glucose, pH 7.4)

http://www.protocol-online.org/biology-forums/posts/25841.html

Krebs buffer (118 mM NaCl, 4.7 mM KCl, 1.2 mM KH2PO4, 1.2 mM MgSO4, 4.2 mM NaHCO3, 2 mM CaCl2, 10 mM glucose, 200 mM sulphinpyrazone and 10 mM Hepes, pH 7.4)

http://www.med.upenn.edu/kaestnerlab/documents/Krebs.pdf

and so on
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