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Topic: How does the methanol react with vitamin b6? Is there any formula I could follow  (Read 9819 times)

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fiestyice2

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Okay, I am doing an HPLC experiment and I am using methanol to extract vitamin b6 from nuts.

How does the methanol react with vitamin b6? Is there any formula I could follow that explains the chemical reaction????
argh! thanks!
« Last Edit: May 16, 2005, 08:00:40 PM by Mitch »

Garneck

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Re:*delete me* HPLC
« Reply #1 on: May 15, 2005, 02:17:16 PM »
There is no chemical reaction.

You'll be extracting vitamin B6 - it will be extracted from the mashed nut mass into methanol. Then, you'll inject the methanol/vitamin B6 sample into the chromatograph, it'll get divided (probably on a reversed phase HPLC, so that methanol leaves the column immediately) and you'll get pure vitamin B6

fiestyice2

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Re:*delete me* HPLC
« Reply #2 on: May 15, 2005, 04:28:09 PM »
What do you mean by extracting the vitamin b6 into methanol?
I am suppose to explain a chemical formula happening in that experiment... since you said there arent chemical reaction, any ideas on what formula I can use to explain?
Thanks!!!

Garneck

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Re:*delete me* HPLC
« Reply #3 on: May 15, 2005, 04:38:43 PM »
Okay, I am doing an HPLC experiment and I am using methanol to extract vitamin b6 from nuts.

What do you mean by extracting the vitamin b6 into methanol?

Ok, so now you don't even know what you're writing.
Please, visit http://www.google.com and read about extractions, since you don't even know what it is.

Offline Mitch

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Re:*delete me* HPLC
« Reply #4 on: May 16, 2005, 08:00:02 PM »
There is no chemical reaction with extractions(typically).  ::)
« Last Edit: May 16, 2005, 08:00:20 PM by Mitch »
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Offline Donaldson Tan

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Re:*delete me* HPLC
« Reply #5 on: May 23, 2005, 09:41:56 PM »
How does the methanol react with vitamin b6? Is there any formula I could follow that explains the chemical reaction????
argh! thanks!

There is no chemical reaction with extractions(typically).  ::)

There is no chemical reaction.

Ok, so now you don't even know what you're writing.
Please, visit http://www.google.com and read about extractions, since you don't even know what it is.

There is no chemical reaction involved here. Vitamin B6 is soluble in methanol. Dissolving is a physical process.
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