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Topic: How to calculate specific activity of LDH?  (Read 9605 times)

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Offline taralini

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How to calculate specific activity of LDH?
« on: February 03, 2010, 08:04:20 PM »
This is what our lab instructor gave us...

Calculate the specific activity of a solution of LDH with the following data. The original solution was diluted 10X, then gave a change in absorbance per minute in the standard enzyme assay of 0.100/min at 340nm. Ten microliters of enzyme solution was added to the assay mixture to give a total assay volume of 3.00 mL. The extinction coefficient for NADH is 0.12 mM. The protein concentration of the undiluted solution was found to be 0.5 mg/mL.

I'm guessing I need to use Beer's Law to find concentration, but I'm not sure where to go from there. *delete me*

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