March 28, 2024, 07:48:18 AM
Forum Rules: Read This Before Posting


Topic: disulfide bonds  (Read 6271 times)

0 Members and 1 Guest are viewing this topic.

stasia222

  • Guest
disulfide bonds
« on: September 29, 2005, 04:57:39 PM »
Hi, I need to know how or what, other than using Glutathione, can break disulfide bonds at a neutral pH.
Thanks
Stacey

Offline lemonoman

  • Atmospheric
  • Chemist
  • Full Member
  • *
  • Posts: 607
  • Mole Snacks: +71/-8
  • Gender: Male
Re:disulfide bonds
« Reply #1 on: October 01, 2005, 02:56:38 PM »
To break the disulfide bond of cystine and form a thiol at each sulfur, they suggest added exces of 2-mercaptoethanol.

See this VERY general mechanism:



from http://www.biochemj.org/bj/357/0343/bj3570343.htm
« Last Edit: October 01, 2005, 03:00:00 PM by lemonoman »

Sponsored Links