Also, Cool Story Bro:
Once had some bubbles in a column I just couldn't shake free and I thought, "Hmmm... Maybe if I put some vacuum on it it will pull those bubble up and out". Let's just say I removed a little more than just the air bubbles.
I also have a lab mate who swears that the best way to get bubbles out of a column is by agitation with a "personal massager". I think the principle is sound, but probably not worth the slew of hostile work environment lawsuits it would precipitate.