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Author Topic: Dry-packing vs slurry-packing: collumn chromotography  (Read 5358 times)

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asa029

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Dry-packing vs slurry-packing: collumn chromotography
« on: August 10, 2011, 11:51:31 PM »

What method of column packing do you prefer and why?

I usually slurry pack my columns. I think its just out of habit more than anything.
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408

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #1 on: August 11, 2011, 02:24:20 AM »

Was taught dry at school.  I use slurry now because it is faster to get the collumn ready.
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Doc Oc

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #2 on: August 11, 2011, 02:55:01 AM »

Slurry.  I tried dry packing once (so my technique was probably off), but I had tons of air bubbles that just wouldn't shake loose and eventually became cracks in the silica so I ended up re-packing the column via slurry anyways.
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Honclbrif

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #3 on: August 11, 2011, 02:56:56 AM »

What J-bone said.

Always slurry pack. You get a better column faster.
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Honclbrif

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #4 on: August 11, 2011, 04:03:18 AM »

Also, Cool Story Bro:

Once had some bubbles in a column I just couldn't shake free and I thought, "Hmmm... Maybe if I put some vacuum on it it will pull those bubble up and out". Let's just say I removed a little more than just the air bubbles.

I also have a lab mate who swears that the best way to get bubbles out of a column is by agitation with a "personal massager". I think the principle is sound, but probably not worth the slew of hostile work environment lawsuits it would precipitate.
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OC pro

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #5 on: August 12, 2011, 07:07:51 AM »

In my Ph.D. times I used to knock at my column while rotating. Was quite effective for removal of air bubbles. With my experience now, it seems to make no effect at all. Just slurry the silica and let the solvent ran through. Maybe help by stirring with a glass rod.
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asa029

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #6 on: August 12, 2011, 09:13:18 PM »

In my Ph.D. times I used to knock at my column while rotating. Was quite effective for removal of air bubbles. With my experience now, it seems to make no effect at all. Just slurry the silica and let the solvent ran through. Maybe help by stirring with a glass rod.

For removing bubbles and for packing the column, I usually apply nitrogen pressure to the top of the column. This works beautifully and i never have problems with bubbles or imperfections in the silica.
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BluePill

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #7 on: September 03, 2011, 12:18:54 AM »

I do what asa029 said. I push N2 into the column and hitting the glass of the column with a tissue roll. It prevents air bubbles.
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408

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #8 on: September 03, 2011, 01:45:08 AM »


I also have a lab mate who swears that the best way to get bubbles out of a column is by agitation with a "personal massager". I think the principle is sound, but probably not worth the slew of hostile work environment lawsuits it would precipitate.

I told one of my friends about this idea when she was doing a collumn this week, who is rather, ummm... uninhibited...and she says she sometimes uses her vibrator for the same thing.   :o :-X ;D
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BluePill

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #9 on: September 03, 2011, 03:38:26 AM »

408, can't stop laughing. :)))))
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asa029

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #10 on: September 03, 2011, 08:28:09 PM »


I also have a lab mate who swears that the best way to get bubbles out of a column is by agitation with a "personal massager". I think the principle is sound, but probably not worth the slew of hostile work environment lawsuits it would precipitate.

I told one of my friends about this idea when she was doing a collumn this week, who is rather, ummm... uninhibited...and she says she sometimes uses her vibrator for the same thing.   :o :-X ;D

Haha, I wonder what my professor would say if I brought a vibrator to the lab to "get the bubbles out". Haha:D
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408

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #11 on: September 04, 2011, 02:23:03 AM »

One way to find out  ;)
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orgopete

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #12 on: October 20, 2011, 03:22:09 AM »

Was taught dry at school.  I use slurry now because it is faster to get the collumn ready.

Slurry, faster.
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karbon

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Re: Dry-packing vs slurry-packing: collumn chromotography
« Reply #13 on: January 03, 2012, 06:53:02 AM »

Sort of a hybrid method, but I usually just put silica in the column, flush some solvent though, add more solvent till its like 3/4th full and shake it.
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