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Topic: UV spectrum  (Read 2731 times)

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Offline mandytan

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UV spectrum
« on: December 06, 2011, 11:09:11 AM »
0.2g of a solid is dissolved in 1L solvent to give a concentration of  0.2g/L.
UV absorbance spectrum was collected.
The solid has 0.1%w/w water content.
What is the formula to calculate the absorptivity on the dried basis? 
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Offline Arkcon

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Re: UV spectrum
« Reply #1 on: December 06, 2011, 01:02:15 PM »
Well, start with the simplest part.  If you mass out 0.2 g of something, and you know it is 0.1 % w/w water, how much did you really add?
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Offline mandytan

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Re: UV spectrum
« Reply #2 on: December 06, 2011, 04:15:24 PM »
So it means I actually added 0.1998g (dried substance).when we calculate absorptivity, do we use the concentration in g/L or mol/L?
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Offline Arkcon

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Re: UV spectrum
« Reply #3 on: December 06, 2011, 04:28:48 PM »
Well, the only info we have so far is grams per liter.  If you have the molar mass, you can convert, depending on what final answer you want.
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