March 28, 2024, 07:53:15 PM
Forum Rules: Read This Before Posting


Topic: Glucose oxidase glucose sensing  (Read 2915 times)

0 Members and 1 Guest are viewing this topic.

Offline vanillafinger83

  • New Member
  • **
  • Posts: 6
  • Mole Snacks: +0/-1
Glucose oxidase glucose sensing
« on: February 28, 2012, 11:52:05 AM »
Hi
I need some help from biochemistry guru for glucose sensing by glucose oxidase.
I'm trying to see if platinum electrode covered with glucose oxidase will detect H2O2 or not with absence of glucose.

1)when you apply (+) voltage to glucose oxidase covered platinum electrode, do you expect it to reduce? in other words, FAD (redox center) in Glucose oxidase will reduce to FADH2 just by applying (+) to conventional three electrode flow cell (RE, CE, wE)?

Help is needed plz help me!! thanx!

Offline Arkcon

  • Retired Staff
  • Sr. Member
  • *
  • Posts: 7367
  • Mole Snacks: +533/-147
Re: Glucose oxidase glucose sensing
« Reply #1 on: February 29, 2012, 09:19:19 AM »
That is not how enzyme using substrate electrodes are constructed.  Basically, the enzyme is immobilized in some sort of polymer, which covers an ion selective electrode, the polymer-enzyme functions as a sort of barrier that affects the interior that then can trigger the electrode.

http://en.wikipedia.org/wiki/Ion_selective_electrode#Enzyme_electrodes
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Sponsored Links