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Author Topic: Preparation of triethylammonium phosphate buffer 1M (TEAP) of pH ~2.8 ?  (Read 35 times)

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How can I prepare the 01M triethylammonium phosphate buffer of pH 2.8~~~~.
I know this can be prepared by using triethylamine (TEA) and phosphoric acid and in my Lab I have Triethylamine and 85% phosphoric acid. I actually search a lot and  I able to find the preparation of TEAA ( triethylammonium acetate buffer of pH ~~~7.4) but couldn't able to find any reference of TEAP buffer.

what I understand from that,  first I have to prepare the 01M triethylamine and I have to adjust the pH of solution to ~2.8 using phosphoric acid?
But I have question when I will add the phosphoric acid the concentration of solution will change and it will not be anymore 1M TEA.

Kindly help me with procedure how to prepare that TEAP buffer?

Thanks in advance.  ::)


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