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Topic: reduction of disulfide to sulfhydryl by NaBH4  (Read 4399 times)

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Offline vioza

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reduction of disulfide to sulfhydryl by NaBH4
« on: February 26, 2015, 01:51:56 AM »
Does anybody had an experience with reduction of disulfide bond to sulfhydryl using NaBH4 in methanol? What conditions are usually required?
The similar reaction is described here Anal Biochem. 2006 Feb 15;349(2):309-11, but there is no procedure associated.

Thanks for help in advance!
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Offline Irlanur

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Re: reduction of disulfide to sulfhydryl by NaBH4
« Reply #1 on: February 26, 2015, 07:06:34 AM »
Do you need to do it with NaBH4? Usually DTT or TCEP are used for this purpose.
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Offline Furanone

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Re: reduction of disulfide to sulfhydryl by NaBH4
« Reply #2 on: February 26, 2015, 11:55:26 AM »
I agree with Irlanur -- DTT or I use mercaptoethanol to more specifically reduce disulfide bonds. Sodium borohydride is a strong reducing agent that will even reduce sugars to sugar alcohols (turn glucose to sorbitol)
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