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Topic: Solvent and apparatus for DNA melting analysis by spectrophotometry  (Read 2020 times)

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Offline Babcock_Hall

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A student whom I know wishes to do some DNA melting experiments, following them by UV/VIS spectrophotometry.  What sort of solvent should she use for a recirculating bath?  I have used ethylene glycol/water for low temperature work, but I have not done much high temperature work.  So far I have not been able to turn up a good techniques-based article.

Offline Yggdrasil

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Re: Solvent and apparatus for DNA melting analysis by spectrophotometry
« Reply #1 on: August 25, 2015, 05:25:41 PM »
For very high temperature applications, silicone oil a good choice for heating baths (up to ~ 200oC, though I suspect you won't need to go that high).  You'd probably be able to go up to at least 90oC with water or ethylene glycol/water, though I'd check the manual for your recirculating bath for recommended solvents.

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