[chonchon430]

Please help...

What is the extinction coefficient of Myoglobin(Equine) at 280nm and 410nm respectively???

THZ!!!

[Yggdrasil]

For any protein for which you know the protein sequence, you can estimate the extinction coefficient from the amino acid sequence using a site like http://web.expasy.org/protparam/

You'd probably have to look up the extinction coefficient at 410 nm, however.  It's likely similar to that of myoglobin from other species, so you may be able to go with those values.

[Babcock_Hall]

I agree about the use of myoglobin from other species.  My only additional comment is that the A₂₈₀ of myoglobin will be affected not only by the presence of Trp and Tyr residues, but also possibly by the heme ring.  Obviously the heme prosthetic group is responsible for a strong absorption is in the area of 410-420 nm or so, but I am not sure about the rest of the spectrum.

[Corribus]

Porphryins have reasonably strong absorption across broad portions of the UV region from N, L, M bands.