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Topic: EDTA solution in acetone or methanol  (Read 5745 times)

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Offline Lily

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EDTA solution in acetone or methanol
« on: March 31, 2016, 05:23:33 AM »
Hi guys.

I need to prepare a non-aqueous EDTA solution preferable in acetone or methanol. Unfortunately, EDTA is slightly soluble in acetone. I tried to heat it and to adjust the pH with an organice base Can somebody help me out with an advice for my problem.
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Offline Arkcon

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Re: EDTA solution in acetone or methanol
« Reply #1 on: March 31, 2016, 05:30:44 AM »
Hmmm ....tricky.  I didn't know if it was possible at all.  So I did a Google search, and my search led right beck to our forum  ;D

http://www.chemicalforums.com/index.php?topic=57846.0

Try to work with the info in that thread.
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Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline Lily

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Re: EDTA solution in acetone or methanol
« Reply #2 on: March 31, 2016, 05:55:49 AM »
Thanks for the quick answer.  :) The problem is that the solution has to be almost complete free of water.  Its for bone histology. I need this solution to decalcify bone sections with an afterwards fixation of the sections in acetone. So I thought of combining the two steps with an EDTA/Acetone solution.
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