I have completed an experiment where I used the changing the concentration of the quencher (Q) method and the changing the concentration of the surfactant (S). The measurements for ln(I0/I) was plotted against [Q] for one graph and got a formula of y=mx +c. Similarly, ln(IO/I) was plotted against
and I also got a graph with the linear formula y=mx +c. Therefore knowing the Slope and y-intercept and given the value of CMC, how do I determine the mean aggregation number for each method? I have the equation
ln(I0/I)=([Q]N)/(0-CMC) but I don't know what values correspond to the slope and y-intercept of the graph which I have created with my experimental data.
Hopefully that makes sense and someone can help