[joe900]

Hello! i will use NMR spectroscopy to determine host-guest binding, and i would like to kindly ask you, what technique is the best to perform NMR titrations!! I probably should prepare solution of guest, and add in portions cavitand into it (also as solution, in the same solvent, e.g. deuterated water)... is it reasonable to prepare mixture of guest with cavitand every time again? like preparation of host-guest, so i: take some part of solution nr 1 (guest solution, prepared for 10 trials for different ration, which volume is constant), add some part of solution nr 2 (cavitand solution, prepared for several trials, which volume changes - 0.5 eq, 1 eq, 1.5 eq with respect to guest), at the end add volume of deuterated solvent to have constant volume of mixture in NMR tube. Would it be right?

[lb2]

Maybe you can find some useful information in one of the standard reviews on the subject: Tetrahedron, 2000, 6151.