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Topic: use of ninhydrin  (Read 2117 times)

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Offline cisz

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use of ninhydrin
« on: September 22, 2016, 05:57:47 AM »
I ran a reaction using urea, imidazole, and n-acetyl cysteine using a microwave oven to produce n-acetyl cysteine amide.

I did tlc using urea, imidazole, NAC, and NAC amide (product as spots.

From what I've read I thought that ninhydrin would be able to make visible spots on tlc plates but, so far, on heating with a heat gun the only spot appearing is the NAC.

Help appreciated.

Offline AWK

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Offline Babcock_Hall

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Re: use of ninhydrin
« Reply #2 on: September 23, 2016, 10:19:36 AM »
I am surprised that ninhydrin was successful, but I have been surprised before.  There is a TLC spray based on Ellman's reagent, which will detect thiol groups.


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