I have a question regarding the protocol of ELISA kit I am using.
It's a sandwich ELISA. After adding samples and standards, the protocol asks to "remove the liquid of each well, don't wash"
how should I remove the liquid? should I use the pipette? should I snap the plate onto absorbant paper?
the next step is to add detection reagent and then to wash. would the expermint be affected if there was some liquid left from the previous step?
I would appreciate your help.