I am new in studying enzyme kinetics and I have some difficulties in analyzing experimental data. I have an experiment of substrate metabolism in the presence and absence of an inhibitor. From the experimental data, I have concentration of substrate vs time as parameters. My question is how I can get from concentration to velocity in order to make after the Lineweaver–Burk plot and to determine Km, vm and the inhibition type. The velocity depends on the enzyme concentration? It should, but then how I calculate the velocity? Is there any formula? I know that the velocity is Δ concentration / Δ time, but I don t want to make a wrong approximation using a line instead of a curved line.
As an example, I have these experimental data:
time(min) concentration (μM)
If I put them on a grafic, the profile is decreasing exponentially.
Thank you for any help or any suggestion!