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Author Topic: How to discover lukewarm (LKW) leucine concentration  (Read 655 times)

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mmrn

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How to discover lukewarm (LKW) leucine concentration
« on: January 17, 2017, 03:00:57 AM »

Hi!!
In the lab I'm working there is a vial containing a lukewarm (LKW) leucine solution that was given from another lab, but I don't have any information about the concentration or anything! I asked around and no one had this written. Is that a way to find out the concentration?  ???
I don't know if it helps but the lab has a scintillation counter. I'll need this to measure bacterial production by the 3H-leucine incorporation into proteins.
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Babcock_Hall

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Re: How to discover lukewarm (LKW) leucine concentration
« Reply #1 on: January 17, 2017, 03:44:25 AM »

I assume that leucine is the only amino acid present.  If you need the concentration of leucine, then you might be able to obtain it colorimetrically, with a quantitative ninhydrin assay.  The other possibility that comes to mind is to analyze for nitrogen in some way.  However, I don't know how the presence of tritium changes the practicality of either experiment.
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