Hi all,
It has been a while since I've studied polarity and charges. Our lab did capillary electrophoresis on sulphates and used malonic acid as an internal standard.
My question is, does malonic acid ionize in the solution with an anionic electrolyte? Does malonic acid have an overall charge? We used indirect method targeting anions.
Sulphate I understand to be an anion, and smaller than malonic acid, so it will migrate quicker.
Other than that, I feel like I have a firm grasp on Capillary electrophoresis - it is just a matter of characterizing the peaks caused by the matrix.
TIA