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Topic: Quantitative measurement of H2O2 in ethanol  (Read 2407 times)

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Offline vinsengho

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Quantitative measurement of H2O2 in ethanol
« on: November 05, 2017, 11:17:35 PM »
Hello.

I'm working on some sort of catalyst under a basic alcoholic solvent. I suspect that some generation of hydrogen peroxide occurs, so I'd like to first confirm the presence of hydrogen peroxide, then perform some sort of quantitative experiment.

I've thought of using titrations. KMnO4 is probably out of the question because it reacts intensely with the alcohol. So iodometric titration perhaps? Any suggestions appreciated, thanks!

Offline wildfyr

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Re: Quantitative measurement of H2O2 in ethanol
« Reply #1 on: November 06, 2017, 04:22:28 AM »
The horseradish peroxidase assay is quite useful for measuring H2O2. A researchgate fellow says its pretty stable in ethanol https://www.researchgate.net/post/How_stable_is_Horseradish_Peroxidase_HRP_in_alcoholic_solutions. The chemicals involved are not expensive. Here is an example procedure (http://eylabs.com/horseradish-peroxidase-enzyme-activity-assay-procedure/) but I think there are several different dyes that can be used, such an aminoantipyrene.

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