[Bozhyte]

In Plasmid (of 2686 base pairs) in 37 C degrees were found that average of 54.3 base pairs were disengaged/disconnected. Find average energy of one hydrogen bond between base pairs. Do not pay attention to π - π interaction.
The formula I have to use is -kT ln (Q^N)
Q means ratio/proportion of interrupted and formed bonds/connections.
K mans Boltzmann constant.
Sorry for my English, would really appretiate *delete me*

[Enthalpy]

Welcome, Bozhyte!

You have a proportion of broken bonds and a temperature. You even have a formula, which is just Boltzmann's distribution slightly rewritten. What are your thoughts?

Boltzmann's distribution tells that even if an event needs more energy than kT, over a big amount of molecules, this event is observed with some frequency, because heat is a mean energy that fluctuates at microscopic scale.

Keep in mind that the small kT relates with molecules and the big RT with moles.

[Bozhyte]

Well I am a bit confused cause I did not know if I should use only this 1 formula and I couldnt even find it on the Internet. Also this problem gives 8 points in work which is a lot so thats why im not sure if its the only thing I have to do.
If I understand it correctly, Q is 54.3/2631.7 = 0.021 ?
Not sure about the N