Hey guys, i'm trying to create a standard curve as a way of measuring the pureness of antabuse.
Great. Why not show us the curve you have.
So far I have used copper(2) complex and antabuse in 80% ethanol without much success.
You shouldn't say a protocol doesn't give much success, if the only thing wrong is a poor curve. Or do you mean it doesn't work? And exactly why not?
I get a curve,
Great.
but the absorbance changes in a negative matter over time,
Bummer. Are you sure that's not supposed to happen? Will is slow down if you wait a while? Will absorbance be gone completely if you wait too long? Then work faster. Or figure out how fast you can perform all tasks, add a minute, and make sure every reading waits exactly that long.
any suggestions on how I could come around this issue?
Try anything. Try everything. Or give up. There's always options.