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Chromatography used in medicinal plant science

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Chantelle C.:
Hi, in our one exam example we have a question regarding which chromatography procedure will be used for a large sample size as in 400g or more no other information is given please help.

jeffmoonchop:
Assuming the sample is homogeneous, you dont need to analyse the whole sample. Just taking a few small samples from the batch will give you confirmation it is what it is. Run all the usual analyses on 6 smaller samples taken after mixing the large sample to ensure precision. I dont know of anyone who would run 400g sample in a chromatography method. If you have to use it all, dissolving it in a large flask would give you true values, but again you would only use around 1ml in a HPLC vial.

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