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Author Topic: photometric determination of urea  (Read 381 times)

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skunny

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photometric determination of urea
« on: December 01, 2018, 03:50:19 AM »

hi everybody

i was hoping somebody could help me with my anaysis. I wanna to a photometric determination of urea. i found a paper, they were working with 4-dimethylaminobenzaldehyde which reacets with urea to a colored compoud. the absorbation maximumist 425nm. They were also using trichloroacetic and I don't know why or what its good for. I already did some research, but i couldnt find an answer. I was hoping if somebody could tell me what it is good for and whether i could substitute it oder leave it out !

thank u=)
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chenbeier

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Re: photometric determination of urea
« Reply #1 on: December 01, 2018, 04:59:26 AM »

It  is a special method to dertermine urea in milk, blood and urine. The TCA is used to precipitate proteine, which disturbes the reaction.

Chapter 8.3 can find

https://fssai.gov.in/MilkSurvey/lab%20parameters/Urea.docx

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skunny

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Re: photometric determination of urea
« Reply #2 on: December 01, 2018, 08:36:52 AM »

Thank you! So I can just leave the tca out? because I do not have any proteins in my sample and you do have proteins in milk or blood or urine
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