Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: NewtoAtoms on April 05, 2009, 11:43:13 AM
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A pentapeptide was found to contain the amino acids Phe, Gly, Met, Ala, Ser.
N-terminal analysis yielded the PTH derivative of alanine.
Partial hydrolysis of the pentapeptide yielded the dipeptides (Gly-Ser)(Ala-Gly)(Ser-Met)
The peptide sequence is:
a. Ala-gly-Ser-Phe-Met
b. Ala-Gly-Ser-Met-Phe
c. Phe-Ser-Gly-Met-Ala
d. Phe-Met-Ser-Gly-Ala
e. Phe-Ala-Gly-Ser-Met
Now I am a TAD bit confused in this question as I dont know where to place the Phe.
I have done the leg work so please let me explain:
The N-terminal analysis yielded the PTH derivative of alanine, therefore Ala, would be located on the far left.
Sequencing the amino acids uncovers :
Ser - Met
Gly - Ser
Ala-Gly
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Ala-Gly-Ser-Met
However where on earth do I put the Phe??
I have thought this through immensely and have come to these conclusions:
a. Ala-gly-Ser-Phe-Met NO because it isn't in the partial hydrolysis dipeptide sequnces
b. Ala-Gly-Ser-Met-Phe PERHAPS but it doens't show the Phe in the sequencing....
c. Phe-Ser-Gly-Met-Ala NO OUT OF ORDER
d. Phe-Met-Ser-Gly-Ala NO OUT OF ORDER
e. Phe-Ala-Gly-Ser-Met THIS IS THE CORRECT ANSWER, because it isn't in the sequencing, the Phe was removed first in the previous PTH derivative????
any help would be GREATLY appreciated!
Newtoatoms
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I'd actually suggest that you have this the wrong way around, and that b is the correct answer.
You've shown that Ala is the N-terminus, and that this is connected to Gly, which in turn is connected to Ser and then Met, so what's left is the Phe.
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1.
The N-terminal analysis yielded the PTH derivative of alanine, therefore Ala, would be located on the far left.
That's definitive; so, you cannot put Phe before Ala.
2.
Sequencing the amino acids uncovers :
There is no sequencing done, they just give you some peptides resulting from hydrolysis (which clearly are NOT all the products from the hydrolysis, as they lack Phe)