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Dilution help
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Mbradley62:
Hello! I have a TNF-a stimulation that has a stock concentration of 10ug/mL. I want to have a final concentration in my 100uL well of 10ng. I have no idea how to solve for the amount of PBS I need to add to the stock so that after I add to the well it is at the 10ng concentration. Any one can help explain?
Hunter2:
Convert 10 μg/ ml to ng/μl to have the same unit like 10 ng/100 μl
How much is it.
Mbradley62:
1 want to add between 1-8ul to the well
Hunter2:
10 μg/ml = 10 ng/ μl
To get 10 ng/100 μl you have to add 1 μl of the stock to 99 μl of the solvent.
Babcock_Hall:
When I teach dilutions, I suggest to students that they draw a picture of what is going on. With respect to your example, I suggest starting with the idea that the mass of material in the pipet tip (1) is equal to the mass of material in the well (2). Putting this idea into a formula: Mass1 = Mass2. The mass of TNFα is equal to the concentration of a solution times the volume of the same solution. Try writing an equation that expresses this idea. Making a picture minimizes plugging incorrect numerical values into a formula.
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