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Topic: Pipetting  (Read 4085 times)

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Offline Shadow

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Pipetting
« on: August 21, 2013, 05:22:13 AM »
How does pipetting work? Or more precise:
Why won't the liquid fall from the glass tube if we seal one end?

Offline curiouscat

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Re: Pipetting
« Reply #1 on: August 21, 2013, 05:27:44 AM »
How does pipetting work? Or more precise:
Why won't the liquid fall from the glass tube if we seal one end?

If you had a long enough pipette it just might.. :)

Offline Arkcon

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Re: Pipetting
« Reply #2 on: August 21, 2013, 09:49:44 AM »
Simple really: http://en.wikipedia.org/wiki/Pipette
Quote
Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid.

You ought to have tried that sort of search for yourself.
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Offline Corribus

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Re: Pipetting
« Reply #3 on: August 21, 2013, 10:16:11 AM »
Random thought of the day: I hate the phrase "partial vacuum". What the heck does it mean?

What men are poets who can speak of Jupiter if he were like a man, but if he is an immense spinning sphere of methane and ammonia must be silent?  - Richard P. Feynman

Offline Archer

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Re: Pipetting
« Reply #4 on: August 21, 2013, 10:35:08 AM »
Random thought of the day: I hate the phrase "partial vacuum". What the heck does it mean?

I though that it means you have a serious leak :)
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Offline Arkcon

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Re: Pipetting
« Reply #5 on: August 21, 2013, 11:13:03 AM »
Random thought of the day: I hate the phrase "partial vacuum". What the heck does it mean?

I though that it means you have a serious leak :)

Oh lawd.
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline curiouscat

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Re: Pipetting
« Reply #6 on: August 21, 2013, 11:29:42 AM »
Random thought of the day: I hate the phrase "partial vacuum". What the heck does it mean?

Is it any worse or more ambigious than "high pressure" or "low temperature"?  ;D

Guess it depends on context.

Offline Corribus

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Re: Pipetting
« Reply #7 on: August 21, 2013, 11:43:26 AM »
Is it any worse or more ambigious than "high pressure" or "low temperature"?  ;D
I think so. At least high or low has a useful relative meaning. For one thing I'd argue an "absolute" vacuum is physically impossible, so all "vacuums" are "partial".  On a more philosophical side, a vacuum is a state of being, and how can you have a partial state of being?  To me it sounds a bit like describing someone as "partially alive". You're either alive or your dead. You either have vacuum or you don't.  What a person really means is that the pressure in the pipette is lower than some external reference point.

I know, it's a point of semantics because we all understand what is meant. But still... I find the phrase inexplicably annoying. :)

Sorry, didn't mean to derail the thread.
What men are poets who can speak of Jupiter if he were like a man, but if he is an immense spinning sphere of methane and ammonia must be silent?  - Richard P. Feynman

Offline curiouscat

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Re: Pipetting
« Reply #8 on: August 21, 2013, 11:56:43 AM »

Sorry, didn't mean to derail the thread.

Pshaww. Don't worry. You probably made it more interesting by derailing it.  :)

I didn't fancy the idea of typing out an answer to the OP that was very eminently googleable in any case.

Offline MrTeo

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Re: Pipetting
« Reply #9 on: August 21, 2013, 12:09:43 PM »
Is it any worse or more ambigious than "high pressure" or "low temperature"?  ;D
I think so. At least high or low has a useful relative meaning. For one thing I'd argue an "absolute" vacuum is physically impossible, so all "vacuums" are "partial".

High vacuum?
 ;D
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Offline Arkcon

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Re: Pipetting
« Reply #10 on: August 21, 2013, 02:47:06 PM »
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

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