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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: bobs on March 23, 2009, 09:39:37 PM

Title: how can i quantify my analytes without having standard
Post by: bobs on March 23, 2009, 09:39:37 PM

I do not have standard for analyte of interest. How can i quantify it in my extract.

Title: Re: how can i quantify my analytes without having standard
Post by: Arkcon on March 23, 2009, 09:45:20 PM

That is going to be a tough one.  Technically, you can't.  At least, some regulatory bodies wouldn't accept data under those circumstances.  You may be able to get useful work from an analogue, or a reasonably similar molecule.  Or you may be able to find a reference, for a very common molecule, that will identify constants you can use to calculate your unknown based on instrument response.

Title: Re: how can i quantify my analytes without having standard
Post by: wpenrose on March 25, 2009, 02:51:45 PM

Sometimes it's possible, but you're going to have to tell us what analytes you're after, and what the matrix is, and what method you're using. For example, a good coulometric analysis equates (time x current passed) exactly to moles.

DB