I am forming methyl-2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidine-5-carboxylate from methyl formate, NaOMe, methyl chloroacetate and 2,4-diamino-6-hydroxypyrimidine. NMR indicates this is successful. I am then hydrolysing with NaOH in H2O:DMSO, and NMR was inconclusive. It is not soluble in anything apart from boiling water. Do I need to neutralise the product, as I assume I am forming the sodium salt?