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Topic: A Dilution Problem, very short : Requires one word answer.  (Read 4322 times)

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Offline istrydummy

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A Dilution Problem, very short : Requires one word answer.
« on: November 17, 2009, 01:14:59 PM »
" ....a suitable blank should be prepared by diluting 1.0 ml of the ' no-substrate- control incubation with 5ml borate buffer( pH9.0)..."

Am i right to assume that the dilution is x 6? Or is it x 5? The wording is troubling me.


Thanks guys.

ps: If further information is required, i can provide.

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Re: A Dilution Problem, very short : Requires one word answer.
« Reply #1 on: November 17, 2009, 01:34:26 PM »
What is the final volume?
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Offline istrydummy

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Re: A Dilution Problem, very short : Requires one word answer.
« Reply #2 on: November 17, 2009, 01:50:07 PM »
10 ml

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Re: A Dilution Problem, very short : Requires one word answer.
« Reply #3 on: November 17, 2009, 01:51:50 PM »
Strange, as I read it - 6 mL. "Dilute with 5 mL" means for me "add 5 mL", plus initial 1 mL that makes 6 mL. But English is not my first language, so I can be wrong.
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Offline sjb

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Re: A Dilution Problem, very short : Requires one word answer.
« Reply #4 on: November 17, 2009, 02:13:49 PM »
Strange, as I read it - 6 mL. "Dilute with 5 mL" means for me "add 5 mL", plus initial 1 mL that makes 6 mL. But English is not my first language, so I can be wrong.

Dilute with 5 ml would mean 5 ml added to me as well (UK English). Dilute to 5 ml would mean a total volume of 5 ml.

Offline istrydummy

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Re: A Dilution Problem, very short : Requires one word answer.
« Reply #5 on: November 17, 2009, 02:40:33 PM »
Thanks guys. Some of my classmates have a x6 dilution but i was in doubt. I think you guys are right, as it says 1ml was diluted by 5ml...

Gracias.

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