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Analyzing protein sample via UV-Vis

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Soulja:
Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

jeffmoonchop:
How about water?

Orcio_87:
Water may not help if the methanol denatured the protein.

Corribus:
What are you hoping that UV-Vis will tell you?

Soulja:
Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

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