Chemical Forums
Chemistry Forums for Students => Organic Chemistry Forum => Topic started by: mobfesk2 on September 02, 2007, 06:52:50 AM
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Hi Chem Forumers!
In an attempt to make 3-sialyllactose, I reacted fetuin (a sialic acid donor) with lactose which hopefully gave me my 3-SL product. I did an NMR on it and found there to be a few impurities of acetic acid (which I can get rid of by freeze-drying).
So, I know I have my product BUT there may also be traces of lactose (the starting material) and free sialic acid. (Fetuin was removed by ultrafiltration). OK, now unfortunately 3 questions arise here :( .
How would I know how much lactose and sialic acid I have besides my product?
Can someone also please explain how I can remove these impurities?
Also, how would I calculate the % yield of my product?
Thank you very much for any suggestions! Much appreciated
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If u want to remove Acetic Acid than neutralize it with proper Base .
Here is the method 2 calculate yield %
Theoritical yield
You have to first calculate M.W of reagents & yield
than Formula for Theoritical yield is = M.W of Reagent/M.W. of yield
Say it to X gram
Practical Yield
Calculate weight of your yield By balancing Machine
Say it to Y gram
Now % Of Yield
% Of yield = Y gram / X gram *100
= -------------- % yield