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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Prozac on May 13, 2010, 06:53:05 PM

Title: Calculating pI values of proteins
Post by: Prozac on May 13, 2010, 06:53:05 PM

Can someone please offer some help on how to find pI(Isoelectric point) values of proteins or point me to a website that offer some step by step help. Lets assume we have the following protein

Pro-Glu-His-Cys

Title: Re: Calculating pI values of proteins
Post by: silentelysium on May 19, 2010, 06:45:54 AM

http://wwwchem.csustan.edu/chem4400/pppI.htm

You need to find out the pKa values of each of the amino acids in your polypeptide.

Hope that helps.

Title: Re: Calculating pI values of proteins
Post by: AWK on May 19, 2010, 07:18:44 AM

Quote from: silentelysium on May 19, 2010, 06:45:54 AM

http://wwwchem.csustan.edu/chem4400/pppI.htm

You need to find out the pKa values of each of the amino acids in your polypeptide.

Hope that helps.

You need to find out the pKa values of free amino and carboxy groups in your polypeptide

Title: Re: Calculating pI values of proteins
Post by: Prozac on May 26, 2010, 07:34:59 PM

 Good link, was very helpful.

Thanks everyone