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Chemistry Forums for Students => Organic Chemistry Forum => Topic started by: watchwrestling on April 14, 2014, 11:49:32 PM

Title: How to find molar absorptivity from a calibration curve?
Post by: watchwrestling on April 14, 2014, 11:49:32 PM
In my lab, we found the following values (x-axis - y-axis):

0.0001 - 0.653
0.000075 - 0.54
0.00006 - 0.446
0.000048 - 0.348
0.00004 - 0.285
0.000035 - 0.25
0.000032 - 0.218

I know we have to use Beer's Law (A = ε l c) but I just can't seem to get the right answer. Our l is 1 cm but I don't know how to find c or A (what exactly are you supposed to use for A - wavelength or absorbance)? Also, I know you have to somehow use the slope from the calibration curve but I don't exactly know what to use. Any help would be greatly appreciated since this is due tomorrow and I'm starting to freak out lol
Update 1: I actually found where I wrote that the wavelength for the stock solution was 504 at 0.944 abs...I don't know if any of this information will help but hopefully it does!
Update 2: Also, we used 3 mL of our stock solution (with a concentration of 0.0020) diluted with varying amounts of water to get the concentrations above.
Title: Re: How to find molar absorptivity from a calibration curve?
Post by: Archer on April 15, 2014, 02:46:00 AM
Firstly you need to put your units in your table.

Then plot the graph.

Then maybe think about the equation of a straight line graph