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Topic: converting ng/ml into micromoles  (Read 13202 times)

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Offline georgiec85

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converting ng/ml into micromoles
« on: September 12, 2008, 10:15:37 AM »
Hi guys, Iv recently started a Biological science based PhD and my chemisty skills need improving.

Im currently struggling to convert ng/ml into micromoles.

I need to convert Insulin (mW 5808) 3.5ng/ml into micromoles.

Can anyone help?

Thanks in advance

Georgie

Offline nj_bartel

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Re: converting ng/ml into micromoles
« Reply #1 on: September 12, 2008, 10:27:56 AM »
http://physics.nist.gov/cuu/Units/prefixes.html

http://alysion.org/dimensional/fun.htm

You just need to know the prefixes from link one.  Read link 2.

However, to solve that problem I believe you need the density of insulin.  -Never mind, misread the units in the problem.  You were given the density, sorry-

Offline Borek

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Re: converting ng/ml into micromoles
« Reply #2 on: September 12, 2008, 10:31:25 AM »
Honestly, this is HS problem but I won't move the question as it would be a cruel joke.

3.5 ng - how many moles? In general - how do you convert mass to moles?

No need for density.

Micromoles doesn't sound like reasonable units, something like picomoles will be more adequate.
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Offline nj_bartel

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Re: converting ng/ml into micromoles
« Reply #3 on: September 12, 2008, 10:37:42 AM »
Oops, you're right; that initial comment came from thinking it just said 3.5 mL insulin.

Offline georgiec85

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Re: converting ng/ml into micromoles
« Reply #4 on: September 12, 2008, 10:46:47 AM »
This is my first post, so I apologise if Iv posted in the wrong place.

Basically I have a stock concentration on insulin (3.5ng/ml) and I need to use 100micromoles for an experiment. In order to work out the dilution factor, I need to do the conversion.

Thanks for your replies. Im in the process of brushing up on my chemistry knowledge, but this is quite urgent.

Georgie

Offline Borek

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Re: converting ng/ml into micromoles
« Reply #5 on: September 12, 2008, 11:34:46 AM »
Basically I have a stock concentration on insulin (3.5ng/ml) and I need to use 100micromoles for an experiment. In order to work out the dilution factor, I need to do the conversion.

So you need 166 cubic meters of the solution. It is a small pool. Are you sure?

100x10-6 mole (or 10-4 mole) times molar mass gives you mass of the insulin. Divide that by 3.5x10-9 to get number of mililiters of solution.
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