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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: jena on September 28, 2005, 11:06:59 AM

Title: Testing purity
Post by: jena on September 28, 2005, 11:06:59 AM

Hi,

My Question:
For testing the purity of a protein which method is suitable?

a. FDNB(N-terminus sequencing)
b. Edman sequence
c. Both are suitable

Answer: c

Is this correct ???
Thank You :)

Title: Re:Testing purity
Post by: sdekivit on September 28, 2005, 04:11:46 PM

seems correct: the N-amino acid is identified with FDNB and Edman sequencing reveals the amino acid sequence of the protein.

Title: Re:Testing purity
Post by: jena on September 29, 2005, 01:12:20 PM

Hi,

What if after running and Edman sequence, you find that the number of proteins that you put in for the experiment gives for example, Lys-Lys-Gly-Glu, does this mean that it produced three proteins or just one ???

Thank You :)

Title: Re:Testing purity
Post by: sdekivit on September 29, 2005, 02:13:21 PM

Quote from: jena on September 29, 2005, 01:12:20 PM

Hi,

What if after running and Edman sequence, you find that the number of proteins that you put in for the experiment gives for example, Lys-Lys-Gly-Glu, does this mean that it produced three proteins or just one ???

Thank You :)

i can't follow you, because you 're talking about peptides and come up with amino acids in a particular sequence.