May 21, 2024, 10:02:11 AM
Forum Rules: Read This Before Posting


Topic: Gas Chromatography  (Read 8180 times)

0 Members and 1 Guest are viewing this topic.

Offline Vitalspace

  • New Member
  • **
  • Posts: 7
  • Mole Snacks: +0/-0
Gas Chromatography
« on: October 13, 2009, 11:30:08 PM »
Describe a method for identifying a compound using gas chromatography analysis.

I am kinda confused on this question...
Thanks!

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 27682
  • Mole Snacks: +1802/-410
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re: Gas Chromatography
« Reply #1 on: October 14, 2009, 03:44:43 AM »
Just read how GC works, even on wiki,general approach is pretty easy.
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info

Offline marquis

  • Full Member
  • ****
  • Posts: 397
  • Mole Snacks: +35/-3
Re: Gas Chromatography
« Reply #2 on: October 14, 2009, 05:47:15 PM »
Normal GC compares a compound to a standard.  If it has a similar retention time, it is called a match.  The compound sensitivity varies depending on the detector (FID, TCD, etc), but the idea is the same.

There are other forms of GC that analyze the mass spectrum  (GC/MS).  Here you have a spectrum to compare as well as a retention time match.  Much better for identification.

Offline bstanley

  • Regular Member
  • ***
  • Posts: 14
  • Mole Snacks: +0/-0
Re: Gas Chromatography
« Reply #3 on: October 15, 2009, 09:02:23 PM »
Describe a method for identifying a compound using gas chromatography analysis.

I am kinda confused on this question...
Thanks!

GC works by retention time. So, let's take an example of compound A, B, C and D. Each compound will have different affinity with the GC column. As a result, they should be well separated and appear as 4 distinct, Gaussian peaks in the chromatogram. Chromatogram is a plot of Intensity (y-axis) Vs time (min or sec). Provided all conditions are the same (temperature, column, gas flow rate, etc.), each compound should have a unique time when it elutes. For example, the peak A may appear at 2 min, B at 3 min, C at 4 min and D at 5 min on the chromatogram. This time associated with each compound is its retention time. If you inject an unknown compound and its retention time is 3 min, then that unknown compound is B (assuming the conditions are the same). Higher concentration will result in taller/bigger peak on the chromatogram, but the retention time will be the same.

Hope this helps.

Sponsored Links