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Topic: Acrylamide Help  (Read 1710 times)

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Offline Jake1013

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Acrylamide Help
« on: February 19, 2014, 06:36:04 AM »
Hi I need to use acrylamide as a control study material. I do no need it for its' separating properties but for it's setting from a solution to gel property in a short period of time.

If I were to buy a 40% Bis/Acrylamide solution and add APS and TEMED to this without the resolving buffer mix (Tris etc) would it still set into a gel? I know I could try this myself but wondered if anyone knew?

Offline Arkcon

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Re: Acrylamide Help
« Reply #1 on: February 19, 2014, 06:57:23 AM »
Yes, I was able to gel it without TRIS or other PAGE additives.
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline Jake1013

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Re: Acrylamide Help
« Reply #2 on: February 19, 2014, 07:28:05 AM »
Thank you

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