Chemical Forums
Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: SpontaneousRxn on January 28, 2010, 12:07:15 AM
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Hi.
I am taking a biochem lab and I was wondering the following:
-for finding the protein concentration of an unknown sample, I am doing a serial dilution and then I am doing a standard with a set of given standards to obtain a standard curve. From the standard curve, I will obtain the absorbancies and therefore the concentration of protein per aliquot. So to find the initial protein concentration, do i just divide by the mL of protein aliquot and multiply it by the dilution factor?
-for an enzyme analysis, i am diluting the enzyme, running a no-enzyme control and from there i will do a spectrophotometric analysis to determine a rate (dA/t). So to calculate the enzyme concentration, do I just get the slope from the rate and divide by molar absorptivity of reactanta and then multiply by aliquot volume and dilution factor and lastly divide by the aliquot used in the spectrophotometric assay???