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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: csinha on February 25, 2013, 05:43:53 PM

Title: determination of moles of unknown gas from calibration curve and peak height
Post by: csinha on February 25, 2013, 05:43:53 PM

I am working on a lab and I am unsure how to get the concentration of three gases in an unknown sample. I was required to create a calibration curve for peak area vs volume of analyte injected. The analyte gas is composed of methyl ethyl ketone, toluene and 2-butanol. The unknown sample contains these same gases. I determined the concentration of moles of each  in the calibration solution by pV=nRT where V is the volume of analyte injected into the GC (50 ul). I do not know how to find the molar concentration of the gases in the unknown sample. I know that peak area is proportional to concentration of an analyte but I do not know how to determine it with the information I have.

Title: Re: determination of moles of unknown gas from calibration curve and peak height
Post by: Borek on February 26, 2013, 03:55:56 AM

If I understand correctly what the problem is - use the calibration curve to determine apparent volume of the sample injected.