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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: Jake1013 on February 19, 2014, 06:36:04 AM

Title: Acrylamide Help
Post by: Jake1013 on February 19, 2014, 06:36:04 AM
Hi I need to use acrylamide as a control study material. I do no need it for its' separating properties but for it's setting from a solution to gel property in a short period of time.

If I were to buy a 40% Bis/Acrylamide solution and add APS and TEMED to this without the resolving buffer mix (Tris etc) would it still set into a gel? I know I could try this myself but wondered if anyone knew?
Title: Re: Acrylamide Help
Post by: Arkcon on February 19, 2014, 06:57:23 AM
Yes, I was able to gel it without TRIS or other PAGE additives.
Title: Re: Acrylamide Help
Post by: Jake1013 on February 19, 2014, 07:28:05 AM
Thank you