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Specialty Chemistry Forums => Biochemistry and Chemical Biology Forum => Topic started by: Memy on April 13, 2006, 06:24:04 AM

Title: hydrophobic interaction chromatography
Post by: Memy on April 13, 2006, 06:24:04 AM

hey

i have just a doubt...when we are talking of  hydrophobic interaction chromatography and reverse phase liquid chromatography, are we talking of the same chromatographic technique?

thank you

Title: Re: hydrophobic interaction chromatography
Post by: Albert on April 13, 2006, 06:36:49 AM

No, they are different. You CAN carry out a HPLC HIC, in order to increase resolution.

HIC gives you the opportunity to use aqueous elution conditions and has a higher capacity.
However, on the other hand, it gives moderate resolution.