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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: Spacenova on December 21, 2015, 02:55:53 AM

Title: UV-VIS measurement
Post by: Spacenova on December 21, 2015, 02:55:53 AM

I need to measure UV assay of a drug substance. I measure standard material 3 times and chcek the calibration linear with a control solution. Then I create average of standards. Should I calculate only from 3 standards or calculate with control as well?

 Thank you.

Title: Re: UV-VIS measurement
Post by: ttn1979 on January 09, 2016, 04:51:22 AM

You should calculate from 3 times of measurement

Title: Re: UV-VIS measurement
Post by: Borek on January 09, 2016, 05:30:40 AM

For me the question is about as ambiguous as the answer.

Title: Re: UV-VIS measurement
Post by: Arkcon on January 09, 2016, 07:41:53 AM

You will have to define what you mean by control better.  Do you mean a separate standard prepared as a check standard, or do you mean bracketing standards, the same prep as beginning standards, that are run after the samples?