Chemical Forums

Specialty Chemistry Forums => Citizen Chemist => Topic started by: CroLifter on September 25, 2019, 09:09:04 AM

Title: Separating organic compound from oily solution
Post by: CroLifter on September 25, 2019, 09:09:04 AM

Hello,

So i am presented with an issue. I received an oily solution which allegedly contains xxx mg of organic compound, esterified hormone in an oily solution (olive oil).

I need to somehow extract that compound and then weigh it on a precision scale, to measure how much of the compound is really inside the solution.

But i have no idea how to go about it. I tried to research online but didnt find anything on pulling the compounds out of oils.


Solution comes in 1ml glass containers.

Help is appreciated

Title: Re: Separating organic compound from oily solution
Post by: AWK on September 25, 2019, 10:11:12 AM

Gel chromatography?

Title: Re: Separating organic compound from oily solution
Post by: wildfyr on September 25, 2019, 02:02:24 PM

I'll expand on AWK's comment, run a silica gel column as described here

https://en.wikipedia.org/wiki/Column_chromatography

It is a technique well suited for separating several nonpolar components.

Title: Re: Separating organic compound from oily solution
Post by: CroLifter on September 25, 2019, 03:20:43 PM

The good thing is that it is only one compound in one solvent.

Title: Re: Separating organic compound from oily solution
Post by: hypervalent_iodine on September 26, 2019, 03:16:28 AM

Is it possible to use analytical techniques to assess how much you have in there? Something like GCMS would be much more precise and accurate than physically extracting and weighing it.